Journal article
Investigation of the Plasmodium falciparum food vacuole through inducible expression of the chloroquine resistance transporter (PfCRT)
F Ehlgen, JS Pham, T de Koning-Ward, AF Cowman, SA Ralph
Plos One | Published : 2012
Abstract
Haemoglobin degradation during the erythrocytic life stages is the major function of the food vacuole (FV) of Plasmodium falciparum and the target of several anti-malarial drugs that interfere with this metabolic pathway, killing the parasite. Two multi-spanning food vacuole membrane proteins are known, the multidrug resistance protein 1 (PfMDR1) and Chloroquine Resistance Transporter (PfCRT). Both modulate resistance to drugs that act in the food vacuole. To investigate the formation and behaviour of the food vacuole membrane we have generated inducible GFP fusions of chloroquine sensitive and resistant forms of the PfCRT protein. The inducible expression system allowed us to follow newly-i..
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Awarded by Australian Research Council Future fellowship
Funding Acknowledgements
This research was supported by research from the National Health and Medical Research Council, Australia (http://www.nhmrc.gov.au/), and the Australian Research Council (http://www.arc.gov.au/). FE was supported by a University of Melbourne postgraduate scholarship, JSP is supported by an Australian Postgraduate Award. SAR is supported by an Australian Research Council Future fellowship FT0990350. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.